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KMID : 0620920230550020385
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Experimental & Molecular Medicine
2023 Volume.55 No. 2 p.385 ~ p.400
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Visuomotor anomalies in achiasmatic mice expressing a transfer-defective Vax1 mutant
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Min Kwang-Wook
Kim Nam-Suk
Lee Jae-Hoon
Sung Young-Hoon
Kim Mu-Seong
Cai-Liang Shen
Kim Jong-Myeong
Kim Jae-Hyun
Lee Jae-Young
Cho Won-Jin
Yang Jee-Myung
Kim Nu-Ry
Kim Jae-Hoon
C. Justin Lee
Park Young-Gyun
Lee Seung-Hee
Lee Han-Woong
Kim Jin-Woo
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Abstract
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In binocular animals that exhibit stereoscopic visual responses, the axons of retinal ganglion cells (RGCs) connect to brain areas bilaterally by forming a commissure called the optic chiasm (OC). Ventral anterior homeobox 1 (Vax1) contributes to the formation of the OC, acting endogenously in optic pathway cells and exogenously in growing RGC axons. Here, we generated Vax1AA/AA mice expressing the Vax1AA mutant, which is incapable of intercellular transfer. We found that RGC axons cannot take up Vax1AA protein from the Vax1AA/AA mouse optic stalk (OS) and grow slowly to arrive at the hypothalamus at a late stage. The RGC axons of Vax1AA/AA mice connect exclusively to ipsilateral brain areas after failing to access the midline, resulting in reduced visual acuity and abnormal oculomotor responses. Overall, our study provides physiological evidence for the necessity of intercellular transfer of Vax1 and the importance of the bilateral RGC axon projection in proper visuomotor responses.
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KEYWORD
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Axon and dendritic guidance, Morphogen signalling
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